Journal: Frontiers in Immunology

Article Title: Sequential therapy with supercharged NK cells with either chemotherapy drug cisplatin or anti-PD-1 antibody decreases the tumor size and significantly enhances the NK function in Hu-BLT mice

doi: 10.3389/fimmu.2023.1132807

Figure Lengend Snippet: Increased susceptibility of differentiated oral and pancreatic tumor cell lines to chemotherapeutic drugs in comparison to their stem-like counterparts. OSCSCs were differentiated using supernatants from IL-2 (1000 U/ml) and anti-CD16 mAbs (3µg/mL) treated primary NK cells as described in Materials and Methods section. OSCSCs, OSCCs and NK-diff-OSCSCs were treated with cisplatin (60 µg/mL) for 18-20 hours, after which, the cells were stained with propidium iodide (PI) to determine percent cell death using flow cytometric analysis (n=3) (A) . OSCSCs and OSCCs were treated with paclitaxel (40 µg/mL) for 18-20 hours, after which, the cells were stained with PI to determine percent cell death using flow cytometric analysis (n=3) (B) . MP2 cells were treated with supernatants from IL-2 (1000 U/ml) and anti-CD16 mAbs (3µg/mL) treated primary NK cells in order to induce differentiation as described in the Materials and Methods section. MP2, PL12, and NK-diff-MP2 cells were treated with cisplatin (60 µg/mL) for 18-20 hours, after which, the cells were stained with PI to determine percentage of cell death using flow cytometric analysis (n=3) (C) . MP2 and PL12 tumor cells were treated with paclitaxel (40 µg/mL) for 18-20 hours, after which, the cells were stained with PI to determine percent cell death using flow cytometric analysis (n=3) (D) . ***( p value 0.0001-0.001), **( p value 0.001-0.01), *( p value 0.01-0.05).

Article Snippet: Propidium iodide (PI) and chromium-51 was purchased from PeproTech (Cranbury, NJ, USA) and Perken Elmer (Waltham, MA, USA), respectively.

Techniques: Comparison, Staining

Journal: Bone

Article Title: AMP-activated protein kinase (AMPK) activation regulates in vitro bone formation and bone mass

doi: 10.1016/j.bone.2010.04.596

Figure Lengend Snippet: Effect of AMPK activators on ROS 17/2.8 cell proliferation. ROS 17/2.8 cells cultured on custom-made plastic slides were incubated in medium containing 2% FCS for 24 h prior to being treated with different concentrations of AICAR (A) and metformin (B) for 24 h. 20% FCS was used as a positive control. Cell proliferation was evaluated by cell staining with the nuclear stain propidium iodide and by counting the total number of cells using an automated “In Cyto” cell counting system. Data shown (mean±SEM, n=6) are representative of two independent experiments. **p<0.01; ***p<0.001, when compared with untreated control.

Article Snippet: Propidium iodide staining solution (PI stain) was purchased from INCYTO (Seoul, South Korea).

Techniques: Cell Culture, Incubation, Positive Control, Staining, Cell Counting, Control